METHOD FOR MEASURING UDP-GlcNAc

PROBLEM TO BE SOLVED: To provide a method for measuring the formation of uridine-diphospho-N-acetylglucosamine (UDP-GlcNAc), inhibition thereof, or the like, in an extract from human cells, animal cells, or the like, contained in a tissue culture. SOLUTION: This method for measuring the UDP-GlcNAc c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	KOCHAN JAREMA PETER, BURGHARDT CHARLES
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMES ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for measuring the formation of uridine-diphospho-N-acetylglucosamine (UDP-GlcNAc), inhibition thereof, or the like, in an extract from human cells, animal cells, or the like, contained in a tissue culture. SOLUTION: This method for measuring the UDP-GlcNAc comprises measuring the activity of glutamine:fructose-6-phosphoroamide transferase. The method includes (a) a step for forming a mixture of the glutamine:fructose-6-phosphoroamide transferase, fructose-6-phosphate, and glutamine, (b) a second step for incubating the mixture obtained in the step (a) under a physiological condition for a time enough to form glucosamine-6-phosphate, (c) a third step for forming N-acetylglucosamine-6-phosphate by acetylating the glucosamine-6-phosphate obtained in the step (b), (d) a fourth step for reacting the N-acetylglucosamine-6-phosphate obtained in the step (c) with Ehrlich reagent, and (e) a step for measuring an amount of the N-acetylglucosamine-6-phosphate existing in the step (d) by measuring an optical density at 500-610 nm of the mixture obtained in the step (d). COPYRIGHT: (C)2004,JPO&NCIPI