METHOD FOR DETECTING HUMAN PARVOVIRUS, AND PRIMER THEREFOR

PROBLEM TO BE SOLVED: To provide a method for mass-producing a human parvovirus (HPV) antigen protein and enabling to measure the HPV or the anti-HPV antibody in a specimen at a high sensitivity. SOLUTION: The specific human parvovirus gene, polypeptide coded by the gene and a system for measuring H...

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Hauptverfasser: MATSUNAGA YASUKO, KUROSAWA DAISUKE, MATSUURA ZENJI, KAMATA KUNIO, SHIMIZU HIDEHARU, YAMAZAKI OSAMICHI, TAKEDA NAOKAZU, OGAWA HIROYUKI
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Sprache:eng
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Zusammenfassung:PROBLEM TO BE SOLVED: To provide a method for mass-producing a human parvovirus (HPV) antigen protein and enabling to measure the HPV or the anti-HPV antibody in a specimen at a high sensitivity. SOLUTION: The specific human parvovirus gene, polypeptide coded by the gene and a system for measuring HPV or anti-HPV antibody are provided. The gene domain containing HPV nonstructural gene and structural gene can obtain a recombinant antigen usable for radio-immunoassay diagnosis, enzyme immunoassay diagnosis or the like by introducing whole sequence or DNA fragments obtained by PCR (polymerase chain reaction) method into a known expression vector, being expressed in host cells transformed by the expression vector. COPYRIGHT: (C)2004,JPO&NCIPI