ASPERGILLUS ORYZAE FLH2 GENE

PROBLEM TO BE SOLVED: To culture a large amount of a new enzyme FLH2 having NO oxidizing action and a new amino acid sequence (416 amino acid residues) by culturing a transformant to E. coli, and to provide a method for easily and simply transforming eucaryotic organisms and screening a transgenic s...

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Hauptverfasser:	ASAI KIYOSHI, KAWATO SHOJI, HATA YOJI, ISHIDA HIROKI, ABE TAKAYOSHI, NAGASAKI HIDEKI, OBATA HIROSHI, GOMI KATSUYA, HOSOYAMA SATORU, AKITA OSAMU, SANO MOTOAKI, KAYA MASAHIKO, OGASAWARA NAOKI, KIN TAISHIN, MACHIDA MASAYUKI, KUHARA SATORU, ABE YASUHISA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	PROBLEM TO BE SOLVED: To culture a large amount of a new enzyme FLH2 having NO oxidizing action and a new amino acid sequence (416 amino acid residues) by culturing a transformant to E. coli, and to provide a method for easily and simply transforming eucaryotic organisms and screening a transgenic strain in introducing the transformant gene to a filamentous fungus by using expression of morphological changes such as coloration of a colony and improvement in spore formation which can be used as a selective marker. SOLUTION: Cloning of the gene coding for the new NO oxidation enzyme FLH2 is carried out. A host (E. coli) is transformed by transducing the new NO oxidation enzyme FLH2 gene into a vector and using an expressed plasmid. The FLH2 protein is confirmed to have maximum absorption at 350-450 nm. Aspergillus oryzae is transformed and compared with the nontrasformed strain to verify performance as the marker for selecting the transgenic strain. COPYRIGHT: (C)2003,JPO