INSECT CELL PRIMARY CULTURE MEDIUM, EXTRACELLULAR MATRIX, AND INSECT CULTURE CELL STRAIN CREATION METHOD IN SHORT PERIOD USING THE SAME

PROBLEM TO BE SOLVED: To provide a new cell culture medium suitable for the primary culture of insect cells, to obtain an insect-originating water-soluble chitin subjected only to deacetylation as a chemical modification agent, and to provide an insect culture cell strain creation method in a short period using the insect cell primary culture medium and the insect-originating water-soluble chitin. SOLUTION: The insect cell primary culture medium contains a lactoalbumin hydrolysate, a yeast extract and a tryptose phosphate broth as protein extracts and polyvinyl pyrrolidone as a viscous auxiliary agent, and the insect-originating water-soluble chitin subjected only to the deacetylation as a chemical modification agent. COPYRIGHT: (C)2003,JPO