COMPOSITION AND METHOD OF GENE ANALYSIS FOR POLYCYSTIC KIDNEY

PROBLEM TO BE SOLVED: To provide a method of testing the gene for identifying the gene alteration relating to the autosomal dominant polycystic kidney or for identifying the nonexistence of such gene alteration. SOLUTION: This invention relates to methods for detection of mutations in a PKD gene usi...

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Hauptverfasser:	AIDAN NOEL HENNIGAN, JIN WAN, SUSAN KIMBERLY ALLEN, KELLY ELLEN FLYNN, ROBICHAUD NORMAND J, JEFFREY GEORGE JONES, JORGE A GARCES, JOHN A CALLAN, CHRISTOPHER M PARATOUCCHI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method of testing the gene for identifying the gene alteration relating to the autosomal dominant polycystic kidney or for identifying the nonexistence of such gene alteration. SOLUTION: This invention relates to methods for detection of mutations in a PKD gene using DHPLC. This invention includes the following aspects: identification of PKD unique sites; design of PKD-specific primers; amplification of PKD-specific products; and analysis of PCR amplified products by DHPLC. The invention further relates to compositions such as identified unique sites and PKD-specific primers, and kits for performing the methods of the invention. COPYRIGHT: (C)2003,JPO