SYSTEM ENABLING HIGH EXPRESSION OF GENE

PROBLEM TO BE SOLVED: To provide a method for stably transferring a target gene into a host organism and expressing the gene at a high rate. SOLUTION: A target gene to be expressed is connected to a pyruvic acid decarboxylase 1 promoter in a yeast Saccharomyces cerevisiae and the product is transferred into the genom of a host to effect the stable expression of the target gene at a high expression rate. In other words, the invention relates to a genetic fermentation method comprising the transfer of the target gene into a genom under the control of the promoter of a gene having an autoregulation mechanism or the promoter of a gene non-essential to the growth or fermentation of the host organism. The promoter may be a DNA having a promoter activity and containing a base sequence of the above promoter wherein 1-40 bases are deleted, substituted, or added, or a DNA having a promoter activity and hybridizable with a DNA having a sequence complimentary to the whole or a part of the base sequence of the above promoter under stringent condition. COPYRIGHT: (C)2003,JPO