SELECTIVE POLYMERASE CHAIN REACTION OF DNA OF WHICH BASE SEQUENCE IS COMPLETELY UNKNOWN
PROBLEM TO BE SOLVED: To provide a method relating to selective polymerase chain reaction of a DNA of an organism, more particularly a method for multiplying the DNA of the organism through polymerase chain reaction even without any information on a primer needed for multiplication of the DNA of the...
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Zusammenfassung: | PROBLEM TO BE SOLVED: To provide a method relating to selective polymerase chain reaction of a DNA of an organism, more particularly a method for multiplying the DNA of the organism through polymerase chain reaction even without any information on a primer needed for multiplication of the DNA of the organism. SOLUTION: Using the method of the invention, whole DNA of the organism of which base sequence is completely unknown can be perfectly multiplied through the polymerase chain reaction. Consequently mutation polymorphism in a base sequence in each mutation is readily analyzed according to the method of the invention by comparing and measuring degree of expression of a mRNA from other samples. |
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