DECARBAMILASE COMPLEX

PROBLEM TO BE SOLVED: To provide a 3D-structural coordinates by obtaining a monocrystal of a complex of decarbamilase and a D-N-carbamoyl-α-amino acid, which is a substrate of the decarbamilase, followed by crystal structure analysis with X-ray, and to provide an excellent decarbamilase isomer which...

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Hauptverfasser: NANBA HIRONORI, NAKAI TAKANAO, MORIKAWA SOICHI
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creator NANBA HIRONORI
NAKAI TAKANAO
MORIKAWA SOICHI
description PROBLEM TO BE SOLVED: To provide a 3D-structural coordinates by obtaining a monocrystal of a complex of decarbamilase and a D-N-carbamoyl-α-amino acid, which is a substrate of the decarbamilase, followed by crystal structure analysis with X-ray, and to provide an excellent decarbamilase isomer which is more advantageous for industrial purpose than the natural decarbamilase by designing a molecule with aims of improving the reactivity against a D-N-carbamoyl-α-amino acid, which is a substrate, optimizing the reactive pH, modifying substrate specificity and others by using the 3D-structural coordinates. SOLUTION: The steric structure of the complex of the decarbamilase and a D-N-carbamoyl-α-amino acid, which is a substrate of the decarbamilase, determined by crystal structure analysis with X-ray. The molecular design technique using the steric structure. A method for obtaining a decarbamilase isomer using the technique. Further, a decarbamilase isomer obtained by the technique.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title DECARBAMILASE COMPLEX
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