POLYGALACTURONASE

PROBLEM TO BE SOLVED: To provide a polygalacturonase that acts on polygalacturonic acid in an exo manner, has an optimal pH around 8, i.e., in an alkaline region, exhibits a protopectinase activity, and is useful as an enzyme used for a detergent for clothes and for treating fibers, and to provide a...

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Hauptverfasser: KAWAI SHUJI, KAIDO YOKO, YAJIMA NORIYUKI, KOBAYASHI TORU, SUZUMATSU ATSUSHI, HIGAKI NORIHIKO, SAWADA KAZUHISA
Format: Patent
Sprache:eng
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Zusammenfassung:PROBLEM TO BE SOLVED: To provide a polygalacturonase that acts on polygalacturonic acid in an exo manner, has an optimal pH around 8, i.e., in an alkaline region, exhibits a protopectinase activity, and is useful as an enzyme used for a detergent for clothes and for treating fibers, and to provide a polygalacturonase gene that permits producing the polygalacturonase as a single product and in a large amount. SOLUTION: A polygalacturonase having a protopectinase activity 30% or more that of the maximal protopectinase at least at a pH from 8 to 11, a microorganism producing the polygalacturonase, a gene encoding the polygalacturonase, a recombination vector containing the gene, a transformant containing the recombination vector, and method for producing the polygalacturonase using these are provided.