ENRICHING DIHALOGENASE-CONTAINING BACTERIUM, METHOD FOR SEPARATING AND CULTURING THE BACTERIUM AND PAPER PRODUCT CONTAINING RESIDUE OF BACTERIUM

PROBLEM TO BE SOLVED: To provide a method for enriching a bacterium containing a dehalogenating enzyme so as to increase individual group of a bacterium to a level effective for dehalogenating a nitrogen-free organohalogen compound present in a polymerization reacrion product, separating the bacteri...

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Hauptverfasser:	BULL ALAN, HARDMAN DAVID J, STUBBS BRIAN M
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE IMPREGNATING OR COATING OF PAPER MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PAPER NOT OTHERWISE PROVIDED FOR PAPER-MAKING PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D PROCESSES USING MICROORGANISMS PRODUCTION OF CELLULOSE PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PULP COMPOSITIONS SPIRITS TEXTILES THEIR PREPARATION OR CHEMICAL WORKING-UP TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G VINEGAR WINE
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Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a method for enriching a bacterium containing a dehalogenating enzyme so as to increase individual group of a bacterium to a level effective for dehalogenating a nitrogen-free organohalogen compound present in a polymerization reacrion product, separating the bacterium and successively culturing the separated bacterium, and to obtain a paper product composed of a nitrogen-containing cationic polymer and the residue of bacterium. SOLUTION: This method comprises (A) adding an environmental sample containing plural solid groups of bacteria to a nitrogen-containing polymer and a nitrogen-free organohalogen compound, (B) incubating the mixed solid groups of bacteria, (C) separating a dihalogenase-containing bacterium from the incubated bacterium solid groups and (D) culturing the dihalogenase-containing bacterium solid group until reaching at least 5×107 cells per milliliter. The paper product comprises (A) a nitrogen- containing cationic polymer and (B) the residue of bacterium present on the maximum of about 100 g based on 1 ton of dried paper product in which the bacterium contains an enzyme capable of dehalogenating the nitrogen free orgenohalogen compound without substantially changing the polymer.