NEW POLYPEPTIDE, DNA ENCODING THE POLYPEPTIDE, AND THEIR USE

PROBLEM TO BE SOLVED: To provide a biological method for hydroxylating position 7 of indole. SOLUTION: Cloning of DNA having a sequence shown by sequence 1 or DNA which hybridizes to the DNA under a stringent condition or the like from a microorganism which has an ability of producing 7-hydroxyindol...

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Hauptverfasser: ARITOKU YASUHIDE, HIROSUE SHINJI, ISSHIKI KUNIO, FUJII TADASHI, YOSHIOKA TAKEO, UEMATSU HITOSHI, WATANABE HIROYUKI
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Sprache:eng
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Zusammenfassung:PROBLEM TO BE SOLVED: To provide a biological method for hydroxylating position 7 of indole. SOLUTION: Cloning of DNA having a sequence shown by sequence 1 or DNA which hybridizes to the DNA under a stringent condition or the like from a microorganism which has an ability of producing 7-hydroxyindole and belongs to the genus Xanthomonas, and providing polypeptides having an enzymatic activity of hydroxylating position 7 of indole, DNA encoding the DNA, a vector consisting of the DNA, and a microorganism carrying the vector allowed producing 7-hydroxyindole and 7-hydroxytryptophan.