AGGLUTINATION, SEPARATION AND RECOVERY OF SPORE BUTRIC ACID BACTERIUM

PROBLEM TO BE SOLVED: To efficiently obtain the subject spore without admixture of various germs by culturing Clostridium butyricum of butric acid bacterium to give a culture solution having formed a spore, mixing the culture solution with an acidic solution of chitosan, agglutinating, separating and recovering the spore. SOLUTION: Clostridium butyricum of butric acid bacterium [e.g. Clostridium butyricum NIP1020 (FERM BP-5794)] is inoculated into a medium, is subjected to static culture at 37 deg.C for 48 hours to form a spore. The culture solution is mixed with an acidic solution of chitosan having >=60% degree of deacetylation and/or >=50 cP viscosity to agglutinate the spore. The spore is separated and recovering from the culture solution to readily and efficiently obtain the objective butric acid bacteria which has putrefaction resisting action and controlling action on intestinal disorder, useful in the field of medicine food, and feed for domestic animals.