METHOD FOR DISSOLUTION OF FIBRIN IN SERUM OR PLASMA SPECIMEN

PROBLEM TO BE SOLVED: To eliminate a measuring error generated when a solid substance existing in a specimen is mixed with the measuring system of a substance to be inspected, by a method wherein a method which dissolves a fibrinous substance in the specimen by using an enzyme is used, and a kit in which a reagent used to dissolve the fibrinous substance is used as a constituent component is used. SOLUTION: A buffer is diluted in such a way that the measured value of a substance to be inspected is not affected and that an enzyme can dissolve fibrin sufficiently. A phosphoric acid buffer at pH 7 or the like in which 1% bivine serum albumin and 0.15 M NaCl are contained is suitable. After that, a enzyme which dissolves fibrin, plasma or an additive which activates plasminogen in plasma and streptokinase are added. This mixture is incubated for a set time, the fibrin is dissolved, and the measurement of the substance to be measured is continued. The amount of the enzyme used for the dissolving reaction of the fibrin can be set at an amount by which the fibrin is dissolved sufficiently as long as a measured result is not affected substantially. That is to say, regarding a serum or plasma specimen, the streptokinase in different amount is added so as to be reacted.