MEASUREMENT OF COMPONENT IN LIQUID SAMPLE BY IMMOBILIZED ENZYME

PROBLEM TO BE SOLVED: To provide a new FIA system capable of preventing reduction in enzyme activity accompanying formation of water-soluble formazan and hardly lowering enzyme activity in an immobilized diaphorase column. SOLUTION: A coloring reaction by immobilized diaphorase is carried out in the...

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Bibliographische Detailangaben
Hauptverfasser:	MAKINO KOICHI, TAKATORI AKIYOSHI, KARUBE MASAO, SHIBAZAKI TOMONORI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROBLEM TO BE SOLVED: To provide a new FIA system capable of preventing reduction in enzyme activity accompanying formation of water-soluble formazan and hardly lowering enzyme activity in an immobilized diaphorase column. SOLUTION: A coloring reaction by immobilized diaphorase is carried out in the presence of a surfactant to prevent deposition of water-soluble formazan. The surfactant is combined with β-NAD+ and used for cleaning the immobilized diaphorase to maintain high enzyme activity. A method for measuring urinary sulfate conjugation type bile acid economically in a high accuracy is provided by an FIA system obtained by combining a column on which BSS and B-HSD are simultaneously immobilized with a mechanism for measuring absorbance before and after an immobilized diaphorase column.