SISTEMA DI CONTROLLO AUTOMATICO PER ELETTROFORESI

PURPOSE: To improve cataphoresis separation by receiving the support of a computer and utilizing a knowledge based on calibration data that are obtained by separating a gigantic molecule with a known size under conditions for optimizing separation. CONSTITUTION: A system 100 consisting of a computer...

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Bibliographische Detailangaben
Hauptverfasser:	BERS GEORGE E, BIRREN BRUCE W, STUBBLEBINE WILL H, LAI ERIC
Format:	Patent
Sprache:	ita
Schlagworte:	CALCULATING COMPUTING COUNTING ELECTRIC DIGITAL DATA PROCESSING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS SEPARATION TESTING TRANSPORTING
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Beschreibung
Zusammenfassung:	PURPOSE: To improve cataphoresis separation by receiving the support of a computer and utilizing a knowledge based on calibration data that are obtained by separating a gigantic molecule with a known size under conditions for optimizing separation. CONSTITUTION: A system 100 consisting of a computer system 150, a contour fixing homogeneous electric field(CHEF) mapper 120, and a cataphoresis device 130 operates in a complete automatic mode. A user inputs the minimum and maximum fragment sizes of a gigantic molecule to be separated, especially DNA. Related knowledge base is provided, thus determining optimized parameters of a forward voltage gradient, a backward voltage gradient, a voltage gradient, an angle, an initial switch time, a final switch time, and a run period. The mapper 120 can directly calibrate the device 130 using the parameters, thus separating the maximum gigantic molecule.