Prodn. of solid culture media

Prodn. of nutrient media for culture of microorganisms or cells of macroorganisms is effected by (a) copolymerising acrylamide (I) and N,N'-methylenebisacrylamide (II) in a (I):(II) wt. ratio of (15-20):(0.019-0.132) in a physiological soln. (III) in the presence of a radical initiator; (b) was...

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Hauptverfasser: GASHINSKY VLADIMIR VLADISLAVOVICH, GOTS JURY DENISOVICH, KOLENSNIKOV MIKHAIL MIKHAILOVICH, PAVLENKO VIKTOR ALEXANDROVICH, MORGUNOV IGOR NIKOLAEVICH, KOKOSHA NATALYA VLADIMIROVNA, KRAINJUKOVA TATYANA IVANOVNA, MAIDANJUK VITALY FEDEROVICH, STEPANENKO VIKTOR IVANOVICH, VOITSEKHOVSKY VALERY GRIGORIEVICH, MARCHENKO NIKOLAI NIKOLAEVICH, BILICH BORIS EVSEEVICH, EGOROV VLADIMIR PETROVICH, KOLYADENKO VLADIMIR GRIGORIEVICH
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Sprache:eng ; ita
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Zusammenfassung:Prodn. of nutrient media for culture of microorganisms or cells of macroorganisms is effected by (a) copolymerising acrylamide (I) and N,N'-methylenebisacrylamide (II) in a (I):(II) wt. ratio of (15-20):(0.019-0.132) in a physiological soln. (III) in the presence of a radical initiator; (b) washing the resulting gel with (III); and (c) either (i) swelling the gel in (III) until its wt. increases by a factor of 2.8-4.5, and then treating the gel with a liq. nutrient medium, or (ii) swelling the gel in a liq. nutrient medium until its wt. increases by a factor of 2.5-3.5.