A METHOD FOR DIRECTIONAL CLONING OF PCR PRODUCTS

The present invention provides a cloning method for directional cloning of PCR fragments with preformed sticky ends. Said PCR fragments can then be directly cloned into a compatible pre-digested vector with corresponding restriction overhangs that are complimentary to the overhangs in the PCR produc...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	AMARINDER SINGH BAWA, KUDUMALA PRAKASH NARAYANA REDDY, MURALI HARISHCHANDRA SRIPATHY, RAMLAL SHYLAJA, BATRA HARSH VARDHAN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a cloning method for directional cloning of PCR fragments with preformed sticky ends. Said PCR fragments can then be directly cloned into a compatible pre-digested vector with corresponding restriction overhangs that are complimentary to the overhangs in the PCR product. The invention also provides for inframe fusion of PCR fragments from two or more distinct genes by generating PCR fragments with sticky ends ligating them and cloning the PCR product into a pre-digested vector.