SUPERCOILED MINIVECTORS AS A TOOL FOR DNA REPAIR ALTERATION AND REPLACEMENT

In some embodiments the present disclosure provides a composition for targeted alteration of a DNA sequence and methods of altering the targeted DNA sequence using the composition. In some embodiments such a composition comprises a MiniVector comprising a nucleic acid sequence template for homology...

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Bibliographische Detailangaben
Hauptverfasser:	HUMBERT OLIVIER, ZECHIEDRICH E. LYNN, FOGG JONATHAN, BAKKALBASI EROL, MAIZEL NANCY, CATANESE JR. DANIEL JAMES
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	In some embodiments the present disclosure provides a composition for targeted alteration of a DNA sequence and methods of altering the targeted DNA sequence using the composition. In some embodiments such a composition comprises a MiniVector comprising a nucleic acid sequence template for homology directed repair alteration or replacement of the targeted DNA sequence within a cell in vivo or in vitro where the MiniVector lacks both a bacterial origin of replication and an antibiotic selection gene and wherein the Mini Vector has a size up to about 2 500 base pairs.