Method and portable device for detection of nucleic sequences in suspected coronavirus samples

Present invention refers to method and portable device(1) for detection and identification of specific sequences of nucleic acids in different samples, by utilisation of reverse transcription technique and/or isothermal amplification, using specific oligonucleotide primers of target region(s) to det...

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Bibliographische Detailangaben
Hauptverfasser: DE LEAO E FLORES Alcino Orfeu, REIMAO PINTO DE FRANCA DORIA Goncalo Maria
Format: Patent
Sprache:eng ; heb
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Beschreibung
Zusammenfassung:Present invention refers to method and portable device(1) for detection and identification of specific sequences of nucleic acids in different samples, by utilisation of reverse transcription technique and/or isothermal amplification, using specific oligonucleotide primers of target region(s) to detect, whereby the amplification of the same is conducted in indirect and non- specific form, or direct and specific, using discriminatory amplification reagents, which signal, colourimetric/fluorescent, is recorded by device (1) where reaction is carried out. Device is controlled by a mobile application(9), also recording data acquired by device (1) analyses, stores it in remote server in cloud (10), which analyses global set of all data allowing global analysis of for example, evolution of epidemics. Invention is useful for detection and identification of relevant nucleic sequences for different types of diagnostic in different clinical, pharmaceutical, veterinary, food, environmental, biotechnological, biosafety areas, particularly useful in quick, low-cost diagnostics, and at point of care, of SARS-CoV-2 virus.