Method of detecting an endotoxin using limulus amebocyte lysate substantially free of coagulogen

The present invention is related to a method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising:(a) contacting the sample with a reagent comprising limulus amebocyte lysate (LAL) and a chromogenic substrate; and(b) measuring a chromogenic effect resulting from a c...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	HERBST David S, NICHOLS Kenneth E, STUMBAUGH Candice
Format:	Patent
Sprache:	eng ; heb
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	The present invention is related to a method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising:(a) contacting the sample with a reagent comprising limulus amebocyte lysate (LAL) and a chromogenic substrate; and(b) measuring a chromogenic effect resulting from a change in the chromogenic substrate in the presence of endotoxin in the sample;wherein the LAL is substantially free of coagulogen. The method also relates to compositions and kits comprising LAL substantially free of coagulogen, and methods of making such.