Expression vectors for enhanced production of polypeptides plasmides containing the vectors hosts containing the plasmids products manufactured thereby and related methods

Disclosed is an improved plasmid which upon introduction into a suitable Escherichia coli host cell containing the thermolabile repressor CI, renders the host cell capable, upon increasing the temperature of the host cell to a temerature at which the repressor is destroyed, of effecting expression o...

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Hauptverfasser: VOGEL TIKVA, OPPENHEIM AMOS, ZEEVI MENA, GORECKI MARIAN, AVIV HAIM, LEVANON AVIGDOR, ZEELON ELISHA
Format: Patent
Sprache:eng
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Zusammenfassung:Disclosed is an improved plasmid which upon introduction into a suitable Escherichia coli host cell containing the thermolabile repressor CI, renders the host cell capable, upon increasing the temperature of the host cell to a temerature at which the repressor is destroyed, of effecting expression of a gene encoding superoxide dismutase inserted into the vector, and production of the superoxide dismutase encoded by the gene.