METODE UNTUK MENGHASILKAN ASAM L-GLUTAMAT MELALUI FERMENTASI

A coryneform bacterium having enhanced intracellular pyruvate dehydrogenase activity which is obtained by increasing copy number of a gene coding for intracellular pyruvate dehydrogenase and having L-glutamic acid-producing ability is cultured in a medium preferably containing vitamin B1 at a concen...

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Bibliographische Detailangaben
Hauptverfasser:	KAZUHIKO MATSUI, TSUYOSHI NAKAMATSU, SOHEI KANDO, EIICHIRO KIMURA, OSAMU KURAHASHI, ISSEI HORINO
Format:	Patent
Sprache:	ind
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A coryneform bacterium having enhanced intracellular pyruvate dehydrogenase activity which is obtained by increasing copy number of a gene coding for intracellular pyruvate dehydrogenase and having L-glutamic acid-producing ability is cultured in a medium preferably containing vitamin B1 at a concentration of 20 mu g/L or higher, so that L-glutamic acid should be accumulated in the medium, and L-glutamic acid is collected from the culture. According to the present invention, a bacterial strain having high L-glutamic acid-producing ability has been bred, and there is provided a method for efficiently producing L-glutamic acid at a low cost.