ENZYMATIC CONVERSION OF PROTEINS USING IMMOBILIZED DICTYOSTELIUM DIPEPTIDYLAMINOPEPTIDASE

The present invention concerns a novel process for using a recently discovered dipeptidylaminopeptidase isolated from the cellular slime mold, Dictyostelium discoideum (dDAP). The claimed process involves immobilizing dDAP onto a support surface so that even numbered, N-terminal dipeptides can be ef...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	FOSTER,LISA KAY, ATKINSON,PAUL ROBERT
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention concerns a novel process for using a recently discovered dipeptidylaminopeptidase isolated from the cellular slime mold, Dictyostelium discoideum (dDAP). The claimed process involves immobilizing dDAP onto a support surface so that even numbered, N-terminal dipeptides can be efficiently removed from precursor proteins or polypeptides to yield desired processed proteins or polypeptides. The invention provides a means for reusing dDAP in multiple conversion reaction runs, as the enzyme does not become a part of the substrate/product solution. The claimed process is particularly useful for converting a precursor human proinsulin analog (Met-Arg-Human Proinsulin having Lys at position 28 and Pro at position 29 of the corresponding insulin B-chain; MR-KPB-hPI) to a processed insulin analog (Human Proinsulin having Lys at position 28 and Pro at position 29 of the corresponding insulin B-chain, KPB-hPI).