PROCESS FOR PRODUCING PEPTIDE ANALOGS IN LARGE NUMBERS, AS WELL AS NEW TYPE PEPTIDE ANALOGS

A method of simultaneously producing a large number of individual C-terminally extended analogs of Leu -VIP is described. Said method comprises the steps of synthesizing two separate mixtures of equally long oligonucleotides coding for N-terminal and C-terminal portions of Leu -VIP analogs, respecti...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	CSERPAN,IMRE,HU, TJOERNHAMMAR,MARIE-LOUISE,SE, SIMONCSITS,ANDRAS,SE, BARTFAI,TAMAS,SE, KALMAN,MIKLOS,HU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS SUGARS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A method of simultaneously producing a large number of individual C-terminally extended analogs of Leu -VIP is described. Said method comprises the steps of synthesizing two separate mixtures of equally long oligonucleotides coding for N-terminal and C-terminal portions of Leu -VIP analogs, respectively, each having a few defined triplets which are made ambiguous and a few identical bases at the 3'-end, mixing said two mixtures to form a mixture of partially double stranded equally long DNA sequences, which are enzymatically converted into double stranded DNA sequences, subjecting the last mentioned mixture to cleavage, with two different enzymes to produce DNA sequences, which are inserted into similarly cleaved vectors by ligation, whereupon the vectors, are transformed into hosts, propagating said hosts to form colonies, which are analyzed one by one to establish the DNA sequences which code for a single protein, propagating separately under expression conditions those hosts which contain vectors having identified protein coding sequences, whereupon the separately expressed proteins are cleaved to release the C-terminally extended analogs of Leu -VIP. Additionally there are disclosed new C-terminally extended analogs of Leu -VIP and plasmids containing genes coding for the new analogs of Leu -VIP.