NANOSENSOR FOR DETERMINING THE CONCENTRATION OF PHYSIOLOGICALLY ACTIVE INORGANIC IONS ON SUBCELLULAR LEVEL

The invention relates to a sensor of formula (I) for determining the concentrations of physiologically active inorganic ions on subcellular level wherein (H) is a silicon dioxide, noble metal or carbon carrier particle with an average diameter of 10-200 nm or a dendrimer with an average diameter of 10-200 nm optionally comprising dye molecules which are selectively sensitive to a physiologically active inorganic ion, A is a secondary antibody, X is the residue of a dye molecule selectively sensitive to a physiologically active inorganic ion, Y is the residue of a fluorescent marker molecule, mis 1-3, n is at least 10, but if (H) is a dendrimer, n may also be 0-9, p is zero or a positive number not exceeding n/10, L1 is a linker of the structure -F1-B-F2-, L2 is a linker of the structure -F1B'-F2, and L3 is a linker of the structure -F'1-B'-F'2-- The invention also relates to methods of preparing and use of the sensor.