NON-DISRUPTIVE DNA ISOLATION FROM CORN SEEDS

This invention relates to systems and process for isolating DNA from biological materials such as seeds while retaining a viable seed for further use. The seed from which the DNA is isolated remains viable and is used or discarded based on the DNA analysis of the seed soak solution. The seed soak so...

Ausführliche Beschreibung

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Bibliographische Detailangaben
1. Verfasser:	HANNAPPEL, Ulrich Stephan
Format:	Patent
Sprache:	eng ; hun
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY FERTILISING FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PHYSICS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PLANTING SOWING TESTING TRAPPING
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Beschreibung
Zusammenfassung:	This invention relates to systems and process for isolating DNA from biological materials such as seeds while retaining a viable seed for further use. The seed from which the DNA is isolated remains viable and is used or discarded based on the DNA analysis of the seed soak solution. The seed soak solution can have substantially all of the confounding maternal DNA from the seed eliminated from the seed soak solutions by employing intact seed pretreatments. This method is particularly useful for maize seed.