A NOVEL NINHYDRIN REAGENT FOR ANALYSING NITROGEN-CONTAINING COMPOUNDS

A NOVEL NINHYDRIN REAGENT FOR ANALYSING NITROGEN-CONTAINING COMPOUNDS

A ninhydrin reagent for use in a method for analysing nitrogen-containing compounds, in particular visualising nitrogen-containing compounds by a colour forming reaction, is provided, the ninhydrin reagent comprising ninhydrin; an aqueous buffer; and a temperature-dependent reducing agent, which age...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Pallot, Michael Gerard, Jones, Philip, Pitts, Leslie John
Format: Patent
Sprache:eng ; hun
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:A ninhydrin reagent for use in a method for analysing nitrogen-containing compounds, in particular visualising nitrogen-containing compounds by a colour forming reaction, is provided, the ninhydrin reagent comprising ninhydrin; an aqueous buffer; and a temperature-dependent reducing agent, which agent is inactive in the reduction of ninhydrin at a first temperature and active in reducing ninhydrin to hydrindantin at a second temperature, wherein the second temperature is higher than the first temperature. The reagent is particularly useful in the analysis of amino acids.