METHOD TO GROW LAWSONIA INTRACELLULARIS BACTERIA IN PERSISTENTLY INFECTED MCCOY CELLS

METHOD TO GROW LAWSONIA INTRACELLULARIS BACTERIA IN PERSISTENTLY INFECTED MCCOY CELLS

The present invention pertains to a method to obtain McCoy cells persistently infected with Lawsonia intracellularis bacteria, comprising infecting McCoy cells with Lawsonia intracellularis bacteria, growing the infected McCoy cells in a suitable medium at an oxygen concentration less than 18% to ar...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: LIJSDONK, Barry, VERDELLEN, Bram, GELDER VAN, Petrus, BIERMANN, Yvonne, Maria, Johanna, Corina, SCHRIER, Carla, Christina
Format: Patent
Sprache:eng ; hun
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The present invention pertains to a method to obtain McCoy cells persistently infected with Lawsonia intracellularis bacteria, comprising infecting McCoy cells with Lawsonia intracellularis bacteria, growing the infected McCoy cells in a suitable medium at an oxygen concentration less than 18% to arrive at a culture of McCoy cells infected with Lawsonia intracellularis bacteria, passing at least a part of the said culture to fresh medium, and without adding uninfected McCoy cells to the medium, growing the infected McCoy cells contained in the said at least part in the fresh medium at an oxygen concentration less than 18%, to obtain the persistently infected McCoy cells. The invention also pertains to the use of such persistently infected cells to grow and obtain these bacteria in purified form.