Method and apparatus for analysing fluorescence of a sample

The invention is a method for fluorescence analysis of a sample in the course of which a fluorescence image is recorded and a fluorescent object identification step is carried out on the fluorescence image. The invention is, furthermore, is an apparatus for fluorescence analysis of a sample, the apparatus comprising a sample holder spatial region (10), an excitation light sources (12a, 12b), a fluorescence detector (14) adapted for recording a fluorescence image by imaging the fluorescent emitted light emitted under the effect of the fluorescent excitation light of a specific analysed sample volume present in the sample holder spatial region (10) during an analysis, and a holographic arrangement adapted for recording holographic images of the specific analysed sample volume, wherein the holographic arrangement comprising a holographic light source (16) and a holographic detector (18). In the apparatus the fluorescence detector (14) and the holographic detector (18) are arranged on a common optical axis with the sample holder spatial region (10) at opposite sides of the sample holder spatial region (10), and the apparatus comprises a coupling-in element (20) adapted for imaging an object wave originating from the holographic light source (16) onto the common optical axis and directing it towards the sample holder spatial region (10), and also adapted for reflecting the light of the holographic light source (16) and transmitting the fluorescent emitted light.