METHOD OF OBTAINING CELL LINES IN A PROTEIN-FREE MEDIUM AND CELL LINES THUS OBTAINED

The invention relates to a method of obtaining mammal cells which are adapted to grow in a medium free of serum and proteins. The inventive method includes a process consisting of two adaptation steps comprising a critical protein concentration interval for each cell line. The invention also relates...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: SVIETA VITORES SARAZOLA, TAMMY BOGGIANO AYO, ROLANDO PEREZ RODRIGUEZ, LUIS ROJAS DEL CALVO, ADOLFO CASTILLO VITLLOCH
Format: Patent
Sprache:eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention relates to a method of obtaining mammal cells which are adapted to grow in a medium free of serum and proteins. The inventive method includes a process consisting of two adaptation steps comprising a critical protein concentration interval for each cell line. The invention also relates to mammal cell lines which grow in a stable manner in a medium free of serum and proteins over at least 40 generations. The invention further relates to clones which express anti-CD6 T1hT and EGFhR 3 antireceptor humanised antibodies and the anti-CD 3 T 3Q chimeric antibody as well as fragments of said antibodies.