GR850679

A specific DNA sequence can be used in the process to obtain human gamma -interferon. The gene is advantageously synthesised in the form of several fragments which are enzymatically ligated to give larger part-sequences which are incorporated into hybrid plasmids and amplified in host organisms. The...

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Bibliographische Detailangaben
Hauptverfasser:	DR EUGEN UHAMANN, DR WOLFGANG ULMER, DR JOACHIM ENGELS, DR MICHAEL LEINEWEBER
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A specific DNA sequence can be used in the process to obtain human gamma -interferon. The gene is advantageously synthesised in the form of several fragments which are enzymatically ligated to give larger part-sequences which are incorporated into hybrid plasmids and amplified in host organisms. The part-sequences are reisolated and then combined to give the complete gene, which is incorporated into a hybrid plasmid, and the latter is expressed in a host organism.