Method for the isolation and purification of nucleotid-activated sugars from biological sources

Method for the isolation and purification of nucleotid-activated sugars from biological sources

Isolation and purificn. of sugar nucleotides (I) from biological sources (i.e. an enzymatic reaction mixt. or a cell extract, where necessary, after removal of insol. cellular components) comprises removal of dissolved proteins by pptn. with alcohol, evapn. to dryness and chromatography of the (I)-c...

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Bibliographische Detailangaben
Hauptverfasser: KRETZSCHMAR, GERHARD, MARQUARDT, RUEDIGER, SEIFFERT-STOERIKO, ANDREAS, MEIWES, JOHANNES, HOERSCH, BRIGITTE
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SPIRITS
SUGARS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Isolation and purificn. of sugar nucleotides (I) from biological sources (i.e. an enzymatic reaction mixt. or a cell extract, where necessary, after removal of insol. cellular components) comprises removal of dissolved proteins by pptn. with alcohol, evapn. to dryness and chromatography of the (I)-contg. residue on silica gel. The new feature is that the residue is taken up in a 1:1-1:10 (by vol.) mixt. of lower alcohol and 0.5-1 M aq. NH4 salt soln., blended with dry silica gel to form a mass with honey-like consistency and applying this to a column of commercial silica gel of appropriate grain size. The column is then eluted, under pressure, with the specified solvent mixt.