System and method for cell and tissue preparation

There is described a system for manufacture of tissue on a manufacturing line having line stations, the system comprising: an expansion controller managing cell expansion of tissue cells forming expanded cells, the expansion controller controlling fluid and pneumatic valves, the fluid and pneumatic valves controlling direction of the fluid pumped between the line stations, the expansion controller monitoring priming and a leading edge of the fluid, the expansion controller configured to be associated with a first of the line stations; a bioreactor incubating the cells, the bioreactor configured to be associated with a second of the line stations; a bioreactor controller including instructions enabling sequencing of gas mixing, agitation, and temperature control of the bioreactor, the bioreactor controller controlling at least one pump that enables movement of media and the cells from a cell reservoir to the bioreactor, and from the bioreactor to a waste area, the bioreactor controller configured to be associated with the second of the line stations; and a supervisor coordinating execution sequencing among the line stations.