A method of transduction

A method of transducing T cells, comprising providing a compressible container including a base, a top arranged substantially in parallel to said base, and at least one flexible wall element extending between said top and said base and defining an internal volume of said compressible container. A population of T cells in a cell processing medium and a transduction agent is added into said internal volume. The base of the compressible container is moved with respect to said top, thereby causing turbulence of the contents in said internal volume, for a first period of time. The step of moving the base with respect to the top may comprise rotating the base about an axis of rotation extending within a horizontal plane defined by the base; said rotating comprising rotating into a first position in which said base forms a first angle with respect to the horizontal plane and rotating into a second position in which said base forms a second angle with respect to the horizontal plane. The first and second angle may be 1 to 45 degrees, preferably 5 to 25 degrees and most preferably 10 degrees. Rotating the base about an axis of rotation within a horizontal plane of the base provides a rocking motion to agitate the contents of the compressible container.