Recombinant pancreatic cancer cell for tracking of exosomes, and use thereof

Provided are a recombinant pancreatic cancer cell for tracking of exosomes, and the use thereof, which belong to the technical field of biomedicine. The recombined pancreatic cancer cell contains a recombination vector, wherein the pHluorin_M153R-CD63 target gene is inserted into the recombination v...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	Peng Ma, Weisong Xu, Tong Wu, Mingbing Xiao, Renjie Lin, Fei Chen, Kunyan Qian, Jie Ji, Xiaopeng Cui
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Provided are a recombinant pancreatic cancer cell for tracking of exosomes, and the use thereof, which belong to the technical field of biomedicine. The recombined pancreatic cancer cell contains a recombination vector, wherein the pHluorin_M153R-CD63 target gene is inserted into the recombination vector. A stable pancreatic cancer cell line is constructed using the recombinant vector in which the pHluorin_M153R-CD63 target gene is inserted; and extracellular exosomes and tail spots released thereby in an extracellular matrix are effectively labeled, and then pathfinding trajectories thereof can be more easily observed, thereby showing the pathogenic behavior of migrated living cells. The visualization problem in the study of the pathogenic behavior mechanism of exosomes is solved; a bright and stable fluorescent tracer substance suitable for a variety of cell culture environments is constructed; the release and intake amounts of exosomes in the migration process of living cells can be monitored; and observation of pathfinding trajectories is achieved.