Indel molecular marker of ultrasonic mutagenesis salmonella typhimurium hisD gene and use thereof
The present disclosure provides an insertion and deletion (InDel) molecular marker of a hisD gene of ultrasonically-mutagenized Salmonella typhimurium (S. typhimurium) and use thereof, and belongs to the field of molecular biology. In the present disclosure, through ultrasonic mutagenesis to S. typh...
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| creator | Bobo Zhao Yaoyong Ji Pengfei Xie Siyu Ruan Xiaoshuang Liu Haile Ma Anqi Zhou Yanling Wu Yunliang Li Yaxuan Xu |
| description | The present disclosure provides an insertion and deletion (InDel) molecular marker of a hisD gene of ultrasonically-mutagenized Salmonella typhimurium (S. typhimurium) and use thereof, and belongs to the field of molecular biology. In the present disclosure, through ultrasonic mutagenesis to S. typhimurium and sequencing, it is found that all InDel mutations occur in a core mutation region of the hisD gene. Therefore, the core mutation region is used as the InDel molecular marker to determine an insertion or a deletion of a gene reverse mutation, and the InDel molecular marker can also be used to analyze a relationship between a sequence of the hisD gene and a function of a protein encoded thereby. |
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In the present disclosure, through ultrasonic mutagenesis to S. typhimurium and sequencing, it is found that all InDel mutations occur in a core mutation region of the hisD gene. Therefore, the core mutation region is used as the InDel molecular marker to determine an insertion or a deletion of a gene reverse mutation, and the InDel molecular marker can also be used to analyze a relationship between a sequence of the hisD gene and a function of a protein encoded thereby.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240821&DB=EPODOC&CC=GB&NR=2612409B$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240821&DB=EPODOC&CC=GB&NR=2612409B$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Bobo Zhao</creatorcontrib><creatorcontrib>Yaoyong Ji</creatorcontrib><creatorcontrib>Pengfei Xie</creatorcontrib><creatorcontrib>Siyu Ruan</creatorcontrib><creatorcontrib>Xiaoshuang Liu</creatorcontrib><creatorcontrib>Haile Ma</creatorcontrib><creatorcontrib>Anqi Zhou</creatorcontrib><creatorcontrib>Yanling Wu</creatorcontrib><creatorcontrib>Yunliang Li</creatorcontrib><creatorcontrib>Yaxuan Xu</creatorcontrib><title>Indel molecular marker of ultrasonic mutagenesis salmonella typhimurium hisD gene and use thereof</title><description>The present disclosure provides an insertion and deletion (InDel) molecular marker of a hisD gene of ultrasonically-mutagenized Salmonella typhimurium (S. typhimurium) and use thereof, and belongs to the field of molecular biology. 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In the present disclosure, through ultrasonic mutagenesis to S. typhimurium and sequencing, it is found that all InDel mutations occur in a core mutation region of the hisD gene. Therefore, the core mutation region is used as the InDel molecular marker to determine an insertion or a deletion of a gene reverse mutation, and the InDel molecular marker can also be used to analyze a relationship between a sequence of the hisD gene and a function of a protein encoded thereby.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Indel molecular marker of ultrasonic mutagenesis salmonella typhimurium hisD gene and use thereof |
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