Indel molecular marker of ultrasonic mutagenesis salmonella typhimurium hisD gene and use thereof

The present disclosure provides an insertion and deletion (InDel) molecular marker of a hisD gene of ultrasonically-mutagenized Salmonella typhimurium (S. typhimurium) and use thereof, and belongs to the field of molecular biology. In the present disclosure, through ultrasonic mutagenesis to S. typh...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Bobo Zhao, Yaoyong Ji, Pengfei Xie, Siyu Ruan, Xiaoshuang Liu, Haile Ma, Anqi Zhou, Yanling Wu, Yunliang Li, Yaxuan Xu
Format: Patent
Sprache:eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The present disclosure provides an insertion and deletion (InDel) molecular marker of a hisD gene of ultrasonically-mutagenized Salmonella typhimurium (S. typhimurium) and use thereof, and belongs to the field of molecular biology. In the present disclosure, through ultrasonic mutagenesis to S. typhimurium and sequencing, it is found that all InDel mutations occur in a core mutation region of the hisD gene. Therefore, the core mutation region is used as the InDel molecular marker to determine an insertion or a deletion of a gene reverse mutation, and the InDel molecular marker can also be used to analyze a relationship between a sequence of the hisD gene and a function of a protein encoded thereby.