Methods and kits for detection of 11-dehydro-thromboxane B2

Methods, compositions and kits for quantitatively determining specified amounts of 11dhTxB2 in microliter to milliliter quantities of a given sample, wherein, the sample is a biological fluid. Specifically, the biological fluid is a quantity of 1 ml or less of urine from a human subject. The methods may be in the form of consolidated assays that can be run in a high throughput, automation format, such as an enzyme-linked immunosorbent assay (ELISA). Further, the ELISA may be modified into a chemiluminescence assay in order to increase sensitivity and linear range and to reduce the reaction time.