RNA Virus detection method

A method for detection of RNA viruses, especially coronaviruses, which virus contains RNA encoding for at least an RNA-dependent RNA polymerase (RdRP) and having a 5' untranslated region and a 3' untranslated region The method comprising (a) providing a cell culture of a cell line that has...

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Bibliographische Detailangaben
1. Verfasser:	Carl Nicholas Mayers
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for detection of RNA viruses, especially coronaviruses, which virus contains RNA encoding for at least an RNA-dependent RNA polymerase (RdRP) and having a 5' untranslated region and a 3' untranslated region The method comprising (a) providing a cell culture of a cell line that has been engineered to encode RNA comprising a first nucleotide sequence corresponding to an antisense nucleotide capable of translating a reporter protein, and a second nucleotide sequence, flanking the first, that corresponds to a 3' untranslated region of the RNA in the virus, (b) adding a test sample to the cell culture and (c) monitoring the culture for detectable output from the reporter. The reporter may be luciferase. The method may be used for testing or screening of antiviral compounds against such RNA viruses, and a method for testing of immunity against such RNA viruses.