Streptomyces clavuligerus

The invention relates to strains of Streptomyces clavuligerus comprising two point mutations in the promoter region of the ccaR gene and an amino acid substitution in the protein product ccaR. The mutations in the promoter are a C to T point mutation at position 48 of SEQ ID NO 1 and G to A at posit...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	Steven Gary Kendrew, Andrew J Collis, Katherine J Honicker, Nicolas Crowhurst
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention relates to strains of Streptomyces clavuligerus comprising two point mutations in the promoter region of the ccaR gene and an amino acid substitution in the protein product ccaR. The mutations in the promoter are a C to T point mutation at position 48 of SEQ ID NO 1 and G to A at position 143 of SEQ ID NO 1. The mutation in the protein is an arginine to tryptophan substitution at position 32 of SEQ ID NO 2. Also described are methods for producing clavulanic acid using the strain.