Genetically-modified bacteria and uses thereof

A genetically-modified bacterium, for example of the class Actinobacteria, and the use ofsuch a bacterium in the bioconversion of a steroidal substrate into a steroidal product of interest is disclosed. The bacterium is blocked in the steroid metabolism pathway prior to the degradation of the polycy...

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Bibliographische Detailangaben
Hauptverfasser:	Laura Fernández De Las Heras, Lubbert Dijkhuizen, Janet Maria Petrusma, Jonathan Knight, Cecilia Kvarnström Branneby
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A genetically-modified bacterium, for example of the class Actinobacteria, and the use ofsuch a bacterium in the bioconversion of a steroidal substrate into a steroidal product of interest is disclosed. The bacterium is blocked in the steroid metabolism pathway prior to the degradation of the polycyclic ring system, and at the steroid side chain degradation pathway. In a preferred embodiment the genetic modification comprises inactivation of the genes kshA1, kshA2, kshA3, kshA4 and kshA5.