Artificial chromosome for retroviral production

Artificial chromosome for retroviral production

This invention relates to a mammalian artificial chromosome ( also known as a MAC, satellite artificial chromosome, SATAC, an artificial chromosome expression system or an ACES) which is stably replicated and segregated alongside endogenous chromosomes in a mammalian host cell for creating more effi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Nina Kotsopoulou, Celeste Pallant, Conrad Vink
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:This invention relates to a mammalian artificial chromosome ( also known as a MAC, satellite artificial chromosome, SATAC, an artificial chromosome expression system or an ACES) which is stably replicated and segregated alongside endogenous chromosomes in a mammalian host cell for creating more efficient retroviral packing cell lines. The artificial chromosome comprises nucleic acid sequences encoding a replication defective retroviral vector particle, preferably derived from HIV-1, HIV-2, SIV, FIV, EIAV or Visna. The retroviral vector particle comprises the RNA genome of the retroviral vector particle and genes encoding the gag (group specific antigen or HXB2 790-2292), pol (DNA polymerase) and env (viral envelope, GP160, GP120, GP41 or envelope glycoprotein) proteins. Further embodiments of the invention provide mammalian cells comprising such artificial chromosomes and methods for their use in producing retroviral particles.