Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde

A method of processing a specimen in a liquid-based cytology preservative comprising formaldehyde, the method comprising combining the specimen with a protease enzyme (proteinase K) and a formaldehyde scavenger (2-imidazolidone), incubating the reaction mixture at an elevated temperature of 85-100 o...

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Bibliographische Detailangaben
Hauptverfasser:	Brett Wolfe Kirkconnell, Deborah Christine Jensen, Timothy Joseph Wilson
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of processing a specimen in a liquid-based cytology preservative comprising formaldehyde, the method comprising combining the specimen with a protease enzyme (proteinase K) and a formaldehyde scavenger (2-imidazolidone), incubating the reaction mixture at an elevated temperature of 85-100 oC, isolating RNA and amplifying the resulting nucleic acid. The incubation temperature may be between 85oC and 95oC. Also claimed is a composition and a kit comprising 2-imidazolidone, proteinase K, EDTA and a pH buffer and an automated system for processing a specimen comprising a pipetting device and a heating element, a reagent vial comprising a formaldehyde scavenger, proteinase K, EDTa and a pH buffer.