Yeast microorganisms genetically engineered to improve isobutanol biosynthesis

Yeast microorganisms genetically engineered to improve isobutanol biosynthesis

A recombinant yeast the expresses either an endogenous or overexpressed endogenous gene of an enzyme that catalyses at least a step in the conversion of pyruvate to isobutanol, and is further engineered to reduce or eliminated an endogenous aldehyde dehydrogenase (ALDH or ALD6) that catalyses the co...

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Bibliographische Detailangaben
Hauptverfasser: JUSTAS JANCAUSKAS, CATHERINE ASLESON DUNDON, RUTH BERRY, STEPHANIE PORTER-SCHEINMAN, ARISTOS ARISTIDOU, DOUG LIES, PETER MEINHOLD, MELISSA DEY, THOMAS BUELTER, ANDREW HAWKINS, MATTHEW PETERS, JUN URANO
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A recombinant yeast the expresses either an endogenous or overexpressed endogenous gene of an enzyme that catalyses at least a step in the conversion of pyruvate to isobutanol, and is further engineered to reduce or eliminated an endogenous aldehyde dehydrogenase (ALDH or ALD6) that catalyses the conversion of isobutyraldehyde to isobutyrate and also an endogenous pyruvate decarboxylase (PDC) that catalyses the conversion of pyruvate to acetaldehyde. The yeast may further have a glycerol-3-phosphate dehydrogenase (GPD) deletion or mutation. The recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms, including Sacchormyces, Kluyveromyces, Pichia, Hansenula, Issatchenkia and Candida. Methods of isobutanol production are also claimed.