Assay for measuring asymmetric methylarginine in a biological sample

The invention relates to a method of determining the presence and/or amount of an asymmetric methylarginine in a sample, the method comprising: (a) contacting the sample with a nitric oxide synthase (NOS) polypeptide in the presence of a detectably labelled species under conditions which permit the...

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Bibliographische Detailangaben
Hauptverfasser: JAMES MITCHELL LEIPER, PATRICK JOHN THOMPSON VALLANCE, DAVID EDWARD WILLIAMS
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention relates to a method of determining the presence and/or amount of an asymmetric methylarginine in a sample, the method comprising: (a) contacting the sample with a nitric oxide synthase (NOS) polypeptide in the presence of a detectably labelled species under conditions which permit the asymmetric methylarginine and detectably labelled species to bind to the NOS polypeptide; and (b) determining the amount or presence of the detectably labelled species bound to the NOS polypeptide