Phage display assay

A method of producing specific binding members for a cell surface antigen, wherein cells with the antigen at their surface and control cells without the antigen at their surface are immobilised on a solid surface, without fixation, and are contacted with a phage display library with blocking of non-...

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Bibliographische Detailangaben
Hauptverfasser:	CATHERINE JANE HUTCHINGS, SIMON LENNARD
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of producing specific binding members for a cell surface antigen, wherein cells with the antigen at their surface and control cells without the antigen at their surface are immobilised on a solid surface, without fixation, and are contacted with a phage display library with blocking of non-specific binding, and a population of phage displaying specific binding members for the cell surface antigen is identified, the method further comprising obtaining nucleic acid which has a nucleotide sequence encoding specific binding members displayed on phage of the population and producing one or more specific binding members for the cell surface antigen by recombinant expression from nucleic acid with the nucleotide sequence or a derivative nucleotide sequence to produce a product specific binding member.