Nucleotide sequences and protein sciences

There is described a nucleic acid sequence encoding a section of Cdc24p (from Saccharomyces cerevisiae), a GEF, which does not substantially alter the interaction of this protein with the subunit of a hetero-trimeric G protein (G#). Mutant nucleic acid libraries and peptides of Cdc24p which alter th...

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Hauptverfasser:	PETER MICHAEL ALJOSCHA NERN, ROBERT ALAN ARKOWITZ
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	There is described a nucleic acid sequence encoding a section of Cdc24p (from Saccharomyces cerevisiae), a GEF, which does not substantially alter the interaction of this protein with the subunit of a hetero-trimeric G protein (G#). Mutant nucleic acid libraries and peptides of Cdc24p which alter the interaction of this GEF and G# are illustrated. The interruption of Cdc24p and G# prevents mating in yeasts but allows vegetative growth. The use of the products to screen for anti-fungal or anti-cancer agents is envisaged. Mutants of the G#, Ste4p, which prevent the interaction of G# and Cdc24p, are illustrated. The binding of the Far1 protein to Cdc24p is described. An assay is described in which the effect of human oncogenes proto-Dbl and Vav on S. cerevisiae with mating defects is assessed. A dbl peptide is also exemplified.