RECOMBINANT PLASMID DNA PVN22, CODING FOR BIOSYNTHESIS OF HUMAN LEUCOCYTE INTERFERON A-I1 AND STRAIN PSEUDOMONAS SP. 31 (PVN22), A PRODUCER OF HUMAN LEUCOCITE INTERFERON A-I1, CONTAINING IT

PCT No. PCT/SU87/00052 Sec. 371 Date Dec. 15, 1987 Sec. 102(e) Date Dec. 15, 1987 PCT Filed Apr. 29, 1987 PCT Pub. No. WO87/06613 PCT Pub. Date Nov. 5, 1987.A recombinant plasmid DVA pVN22 coding the biosynthesis of a human leukocyte interferon alpha -I1 having size of 10.85 t.p.b. and consisting of...

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Hauptverfasser:	JURY IVANOVICH KOZLOV, JURY DMITRIEVICH TSYGANKOV, GRIGORY MIKHAILOVICH DOLGANOV, EVGENY DAVIDOVICH SVERDLOV, VERA ARONOVNA NARODITSKAYA, VIKTOR EMILIEVICH STERKIN, MARINA ALEXEEVNA SKVORTSOVA, ANDREI JURIEVICH CHISTOSERDOV, VITALY LVOVICH JURIN, LJUDMILA VLADIMIROVNA EVDONINA, MARINA REVAZOVNA EREMASHVILI, SERGEI ANATOLIEVICH TSAREV, ALEXANDR YAKOVLEVICH STRONGIN, GALINA SERGEEVNA MONASTYRSKAYA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Zusammenfassung:	PCT No. PCT/SU87/00052 Sec. 371 Date Dec. 15, 1987 Sec. 102(e) Date Dec. 15, 1987 PCT Filed Apr. 29, 1987 PCT Pub. No. WO87/06613 PCT Pub. Date Nov. 5, 1987.A recombinant plasmid DVA pVN22 coding the biosynthesis of a human leukocyte interferon alpha -I1 having size of 10.85 t.p.b. and consisting of the following units: EcoRI-HindIII-a fragment of the plasmid pAYC37 with the size of 9.45 t.p.b., HindIII-EcoRI-a fragment with the size of 1.4 t.p.b. consisting of the following members: a fragment of DNA with the size of 0.25 t.p.b. with the regulatory range of gene D of the phague phi x174 and the first codons of the gene of interferon gene of interferon alpha -I1 with the size of 0.5 t.p.b., a region of the human genome DNA with the size of 0.65 t.p.b.; it has the following genetic markers: genome ApR ensuring resistance against ampicillin, genome SmR ensuring resistance to streptomycin; contains unique regions of recognition of restrictases: Hind III-O; EcoRI-1.4 t.p.b., BamHI-10.82 t.p.b., it is deposited at the collection of culture of microogranisms of the A11-Union Research Institute of Antibiotics and registered under the entry No. 1788I. A strain Pseudomonas Sp. 31 (pVN22)-producer of a human leukocyte interferon alpha -I1 containing a recombinant plasmid DNA pVN22 produced by the method of genetic engineering by introducing said plasmid into bacteria of the genus Pseudomonas, deposited at the collection of cultures of microoorganisms of the A11-Union Research Institute of Antibiotics on 02.04.86 and registered under the entry No. 1788A.