CONSTRUCTION OF ARSENIC RESISTANCE VECTORS FOR THIOBACILLUS FERROOXIDANS

A process for constructing the plasmids pDR416 and pDR420 which contain arsenic resistance genes and which are able to replicate in both T. ferroxidans and E. coli comprises preparing a recombinant of pBR325 or the like and a T. ferrooxidans plasmid, forming a deletion plasmid and inserting arsenic...

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Hauptverfasser:	DAVID RANDLE WOODS, DOUGLAS ERIC RAWLINGS
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FERROUS OR NON-FERROUS ALLOYS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PRETREATMENT OF RAW MATERIALS PROCESSES USING MICROORGANISMS PRODUCTION AND REFINING OF METALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TREATMENT OF ALLOYS OR NON-FERROUS METALS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A process for constructing the plasmids pDR416 and pDR420 which contain arsenic resistance genes and which are able to replicate in both T. ferroxidans and E. coli comprises preparing a recombinant of pBR325 or the like and a T. ferrooxidans plasmid, forming a deletion plasmid and inserting arsenic resistance genes e.g. from R46 by EcoR1 excision and ligation.