UTILISATION D'ANTICORPS ANTI-NS1 DU BTV DANS UNE METHODE DIVA APPLIQUEE AUX VIRUS BTV

In vitro method for differentiating a vaccinated animal or population of animals vaccinated against Bluetongue virus (BTV) of infected animal or a population from animals infected by the BTV from a biological sample from the animal, comprises (a) contacting the biological sample with the protein NS1 of BTV virus, or its fragment of the protein NS1, and (b) revealing antigen-antibody complex formed in (a) between the NS1 protein, or one of its fragments, and an anti-NS1 antibody likely to be present in the biological sample. In vitro method for differentiating a vaccinated animal or population of animals vaccinated against Bluetongue virus (BTV) of infected animal or a population from animals infected by the BTV from a biological sample from the animal, comprises: (a) contacting the biological sample with the protein NS1 of BTV virus, or its fragment of the protein NS1, and (b) revealing antigen-antibody complex formed in (a) between the NS1 protein, or one of its fragments, and an anti-NS1 antibody likely to be present in the biological sample, where the revelation is carried out by a competitive immunoassay method implementing anti-NSI monoclonal antibody or one of its functional fragments, capable of specifically recognizing the protein NS1 of BTV virus, or one of its antigenic fragments, the anti-NS1 antibody is a monoclonal antibody obtained from a culture supernatant of a hybridoma, the hybridoma is obtained by fusion between a myelomatous cell and a mammalian lymphoid cell, the mammal is previously immunized with a composition or an immunogenic compound comprising the protein NS1 of BTV, native or recombinant, or one of its fragments, the anti-NS1 antibody is selected by a process comprising: (i) contacting the test monoclonal test antibodies thus obtained from culture supernatant from the hybridomas with a native protein or NS1 recombinant of the BTV, and (ii) contacting the test monoclonal antibodies thus obtained from culture supernatant of the hybridoma with a native protein or NS1 recombinant of epizootic hemorrhagic disease virus (EHDV); and (iii) selecting the tested monoclonal antibody if specifically recognizes NS1 protein of BTV, or its fragment, and not recognizes the NS1 protein of EHDV. Independent claims are included for: (1) a kit or kit reagents to differentiate vaccinated animals or population of animals vaccinated against BTV of an infected animal or a population of animals infected with BTV by BTV virus, comprising: the protein NS1